Potency Microbial Unicellular Filoplan Chili and Its Inhibitory to Collectotrichum capsici Causal Antraknose Disease on Chili (Capsicum annum L.)

Abstract

Exploration of unicellular microbes can be used as biological control agents. Biological control is an alternative control so that it is safe for human health and the environment. This study was conducted to isolate filoplan unicellular microbes which have antibiosis ability and characterise the secondary metabolite produced to inhibit Collectotrichum capsici in-vitro. This research was conducted at the Departement Plant Pest and Diseases, University of Brawijaya. Microbe isolated from surface of chilies in different age. Isolates microbes were tested for antagonistic potential against C. capsici. Persistence of the potential antagonist under different conditions of pH, temperature and light were also examined. Antagonist test was conducted to confirm antibiosis mechanism. Furthermore, extracts were characterized using pH, light exposure and temperature. The most potential antagonist was identified as isolate C3C with 36.20% inhibition. pH 5, 350C temperature and light exposure of 0% is the best media optimization with the percentage of inhibition 37.5%, 38.2% and 42.3%.