| dc.description.abstract | Context: Eperisone HCl is a muscle relaxant and vasodilator that is related
to how the drug works on the central nervous system and vascular smooth
muscle, works on peripheral centers and has analgesic action. One
method of analyzing single substances from Eperisone HCl is Thin Layer
Chromatography (TLC)–Densitometry. Aims: The purpose of this research
to determinate the Eperisone hydrochloride level in tablet dosage form.
Methods and Material: Using Thin Layer Chromatographic (TLC)–
densitometry method with methanol as solvents, methanol p.a: ammonium
p.a (100:1) as the mobile phase, pre–coated TLC Silica Gel 60 F254 plate
as the stationary phase and followed by densitometric measurements of
their spots at 267 nm. Statistical analysis used: The parameters that
used for the interpretation of analytical method validation results are the
calculation of retention factor (Rf) value, correlation coefficient (r), relative
standard deviation (RSD), and % recovery. Results: This method shows
good selectivity/specificity with Rf value 0.6 ± 0.02. The linearity signal
using 10 standard concentrations of 50 mg L
−1
to 500 mg L
−1
gives a good
linear relationship with r = 0.994 04. Limit of detection and limit of
quantitation are (40.545, 121.637) mg L
−1
respectively. Precision are tested
for three different days and it’s found that the RSD value is 0.8 %.
Accuracy with the results of the % recovery value in various additions is
99.3 % ± 0.9 %. Conclusions: This method was found to be fast, specific,
precise and accurate also can be successfully applied for the analysis of
Eperisone HCl in marketed dosage forms. | en_US |
