Penurunan Jumlah Folikel Primordial di Ovarium Model Tikus Periodontitis Akibat Infeksi Porphyromonas gingivalis

Abstract

Periodontitis is a chronic inflammation in periodontal tissues due to bacterial infection, including P. gingivalis. This bacterium has many virulence factors, one of which is lipopolysaccharide. It is believed that P. gingivalis and its virulence factors can invade and translocate through blood circulation to other organs including the ovaries. This can cause ovarian dysfunction by reducing the number of primordial follicles. This study aimed to determine decreased ovarian primordial follicles in periodontitis rat models due to P. gingivalis infection. This type of research is in the form of laboratory experiments. In this study, female Wistar strain white rats were divided into treatment groups induced with P. gingivalis every three days for 18 days and control groups not induced with P. gingivalis. After 25 days, rats were euthanized and dissected to take ovarian tissue which was then made into preparations. The preparations were observed using a light microscope at 100x magnification to see the distribution of primordial follicles and 400x to count the number of primordial follicles identified. Based on the calculation, the number of primordial follicles in the treatment group was smaller (2,6±2,608) compared to the number of primordial follicles in the control group (12,4±2,408). The calculation of primordial follicles number was then statistically analyzed using the Independent Sample T-Test test which showed significant results. The study concludes that P. gingivalis infection can reduce the number of ovarian primordial follicles in the ovaries of female periodontitis rat models.