Immobilization of Crown Flower (Calotropis gigantea) Protease Using Celite and Dextrin

dc.contributor.author	Witono, Yuli
dc.contributor.author	Azkiyah, Lailatul
dc.date.accessioned	2016-09-05T06:52:58Z
dc.date.available	2016-09-05T06:52:58Z
dc.date.issued	2016-09-05
dc.identifier.issn	2088-5334
dc.identifier.uri	http://repository.unej.ac.id/handle/123456789/76962
dc.description.abstract	Immobilization of crown flower (Calotropis gigantea) protease using celite and dextrin by entrapment method was investigated in this study. The influenced of celite and dextrin at the concentration (6, 8, 10, 15, and 20 g/g) on enzyme production were evaluated. The optimum condition of immobilized crown flower protease was on temperature 55 o C and pH 7.0. Results showed t hat the best concentration is 8 g/g that reached maximum specific enzyme activity was 5.69 x10 -5 Vol.6 (2016) No. 2 ISSN: 2088-5334 U/mg at celite and 3,309.10 U/mg a t dextrin. Immobilized enzyme is more stable than free enzyme based on the change of pH and temperature of environment. These results indicated that celite is better than dextrin for crown flower protease production.	en_US
dc.language.iso	en	en_US
dc.subject	Crown flower	en_US
dc.subject	Calotropis gigantea protease	en_US
dc.subject	immobilization	en_US
dc.subject	celite dextrin	en_US
dc.subject	specific activity enzyme	en_US
dc.title	Immobilization of Crown Flower (Calotropis gigantea) Protease Using Celite and Dextrin	en_US
dc.type	Article	en_US