Immobilization of Crown Flower (Calotropis gigantea) Protease Using Celite and Dextrin
MetadataShow full item record
Immobilization of crown flower (Calotropis gigantea) protease using celite and dextrin by entrapment method was investigated in this study. The influenced of celite and dextrin at the concentration (6, 8, 10, 15, and 20 g/g) on enzyme production were evaluated. The optimum condition of immobilized crown flower protease was on temperature 55 o C and pH 7.0. Results showed t hat the best concentration is 8 g/g that reached maximum specific enzyme activity was 5.69 x10 -5 Vol.6 (2016) No. 2 ISSN: 2088-5334 U/mg at celite and 3,309.10 U/mg a t dextrin. Immobilized enzyme is more stable than free enzyme based on the change of pH and temperature of environment. These results indicated that celite is better than dextrin for crown flower protease production.
- LSP-Jurnal Ilmiah Dosen