• Login
    View Item 
    •   Home
    • UNDERGRADUATE THESES (Koleksi Skripsi Sarjana)
    • UT-Faculty of Pharmacy
    • View Item
    •   Home
    • UNDERGRADUATE THESES (Koleksi Skripsi Sarjana)
    • UT-Faculty of Pharmacy
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    PENGEMBANGAN BIOSENSOR POLIFENOL BERBASIS POLIFENOL OKSIDASE (PPO) DAN 3-M3TIL-2-BENZOTHIOZOLINON HIDRAZAON (MBTH) UNTUK DETEKSI POLIFENOL PAFA PRODUK MINUMAN TEH DALAM KEMASAN

    Thumbnail
    View/Open
    Diah Ayu Maharani - 092210101088_1.pdf (89.62Kb)
    Date
    2013-12-01
    Author
    Diah Ayu Maharani
    Metadata
    Show full item record
    Abstract
    The purpose of biosensor development is for polyphenols detection in beverage tea products especially green tea product easily and efficiently. The polyphenol biosensor has been made by immobilizing 5.0 µ l reagent (500 unit/ml PPO enzyme and 12.0 mg/ml MBTH (1:1 v/v) is mixture) on whatman filter paper. Polyphenol biosensor has been characterized and the results are polyphenol biosensor has response time about 13-18 minutes, has linearity at concentration range of 25-300 ppm, with correlation coefficient (r) =0.997. This polyphenol biosensor has sensitivity about 0.1078/ppm CE, while LOD = 20.601 ppm CE and LOQ = 68.671 ppm CE. Interference or selectivity of the polyphenol biosensor by catechin and vitamin C concentration ratio of 1:3,33 is 6.117% . Biosensor precision parameter comply with RSD 3.111% and the accuracy parameter with recovery about 103.068 %. Biosensor self-live is 8 days when strored on freezer [(-20)-(0) ºC]. This polyphenol biosensor has been applied to some beverage green tea product and this method is as good as Folin-Ciocalteu.
    URI
    http://repository.unej.ac.id/handle/123456789/2022
    Collections
    • UT-Faculty of Pharmacy [1514]

    UPA-TIK Copyright © 2024  Library University of Jember
    Contact Us | Send Feedback

    Indonesia DSpace Group :

    University of Jember Repository
    IPB University Scientific Repository
    UIN Syarif Hidayatullah Institutional Repository
     

     

    Browse

    All of RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Context

    Edit this item

    UPA-TIK Copyright © 2024  Library University of Jember
    Contact Us | Send Feedback

    Indonesia DSpace Group :

    University of Jember Repository
    IPB University Scientific Repository
    UIN Syarif Hidayatullah Institutional Repository