| dc.description.abstract | The purpose of this research is to investigate the effect of catechin Isolate from GMB4 Clone Green Tea on
apoptotic cell death andoxidative stress in the lens of rats with cataract. Cataract Isolate From GMB4 Clone
Green Tea was induced by intraperitoneal injection of 19 µmol/kg sodium selenite to ten day-old Wistar rats.
The neonatal rats were randomly divided into five groups (n=5 in each group): a control group, and four cataractinduction
groups,
treated
with
either
0,
50,
100,
200
mg/kg
catechin
Isolate
From
GMB4
Clone
Green
Tea.
We
performed
slit-lamp bio microscopic analysis, level of GSH and GR, terminal deoxynucleotidyl transferasemediated
dUTP
nick
end
labeling
(TUNEL)
assay
and
immunohistochemistry
for
caspase-3.
Both
eyes
of
all
rats
in
Group
1
did
not
exhibit
cataract
formation.
In
Group
2,
one
out
of
five
(20%)
developed
grade
3
cataracts
and
the
remaining
four
out
of
five
(80%)
developed
grade
4
cataracts.
The
difference
in
exhibited
cataract
in
the
lens
of
all
rats
between
Group
2
and
any
eyes
of
groups
3
or
4
and
5
were
significant
(P
=
0.022,
0,001,
0,001).
The
grade
of
cataract
formation
was
decrease
in
group
3,
4
and
5.
The
mean
GSH
and
Caspase-3
levels
lenses
of
group
II
rats
were
significantly
(P
<
0.01)
lower
than
the
levels
in
Group
I,
Group
III,
Group
IV and Group V. Apoptotic cell death and oxidative stress in the lens that increased following cataract
formation in rats was suppressed by cathechin Isolate From GMB4 Clone Green Tea. | en_US |
