Produksi Ethanol Berbahan Dasar Hasil Dekomposisi Biomasa Tandan Kosong Kelapa Sawit oleh Mikroba Cellulolytic dan Lignocellulolytic

Abstract

A huge amount of lignocellulosic-rich palm oil empty fruit bunch (POEFB) biomass was released during palm oil harvested. Observation showed that Aspergillus niger could grow well in POEFB without any nutrion added. And after 5 days cultivated at 30oC the extracellular enzyme of A. niger was extracted using 1% NaCl. Of about 500 ml crude enzyme was concentrated by ammonium sulphate precipitation at 70% saturation. The precipitates was then dissolved to 50 ml and dialysed against 20mM acetate buffer at pH 5 to remove remaining ammonium sulphate. Four hundred milligrams of powdered POEFB was suspended in 20 ml dialysed crude enzyme for 36 hours hydrolysation at 37oC and 100 rpm shaken. The hydrolyzates was then fermented using Saccharomycess cerrevisae at 30oC for 24 hours. Colorimetric analysis at OD 580nm using QuantiChrom Kit DIET-500 showed that fermentation produced alcohol 0.88%, respectively. Suggested POEFB is an alternative biomass potential than can be used for bioethanol production efficiently.