Rat Diabetic Blood Glucose Level Profile with Stratified Dose Streptozotocin (SD-STZ) and Multi Low Dose Streptozotocin (MLD-STZ) Induction Methods

Abstract

The prevalence of diabetes increased from year to year. The diabetic animal model was developed to study the pathogenesis and treatment in diabetes. The aim of this study was to determine the profile of diabetic blood glucose level in rat using a stratified dose streptozotocin (STZ-SD) and multi-low dose streptozotocin (MLD-STZ) induction methods. 27 Wistar rats were divided into three groups, the control group, SD-STZ group, and MLD-STZ group. The induction of experimental animal was using the method of SD-STZ and MLD-STZ for five consecutive days intraperitoneally. The SD-STZ method using a stratified dose of 40, 35, 30, 25, and 20 mg/Kg of body weight for five days. MLD-STZ methods using a dose of 20 mg/Kg of body weight for five times. The measurement of blood glucose level (BGL) and body weight (BW) in the animal was made before and on days 2, 4, 7, and 14 after STZ induction. The results of this study indicate that the diabetic conditions (BGL ≥ 300 mg/dl) on the SD-STZ induction method was more stable than MLD-STZ method, and no deaths occurred in experimental animals.