| dc.description.abstract | Banana is one of prebiotic resource food which is duitable nutrition for bactery either
bifidobacterium, eubacterium or lactobacillus, but it is not suitable for bad bactery such as
clostridium, shigella, and veillonella. The advantages of prebiotic come from Inulin which is a polimer
compound comes from fructose units.This research aimed to develop a Thin Layer Chromatography –
Densitometry method of inulin analysis in Banana. Separation of inulin from the sample was
performed using Silica Gel F254 with isocratic elution system using acetonitrile : acetone: acetate
acid (5:5:4) as mobile phase and detected by using UV detector at wavelength 366 nm . This method
validation showed a good linearity with correlation coefficient (r) of 0.9979 while the coefficient of
variation of the regression function (Vx0) was 2%. The limit of detection (LOD) and the limit of
quantification (LOQ) of the method were respectively 6,86 ng and 20,59 n,. The mean absolute
recovery of inulin from the simulation sample was 99,50 % ± 1,75 and the method precision was less
than 1,97%. The proposed method applied to the determination of inulin in the banana showed
concentration 2,10% ((w/w). So, the proposed TLC- Densitometry method is rapid, simple, and
selective for routine analysis. | en_US |
