DEVELOPMENT OF VOLTAMMETRIC BIOSENSOR FOR THE SCREENING OF PLANT EXTRACT WITH ANTI ALZHEIMER ACTIVITY

Abstract

Alzheimer's is characterized by involvement of neurotransmitter acetylcholine in Alzheimer’s disease resulting into disproportionate deficiency of acetylcholine by degradation activity of acetylcholinetransferase and acetylcholinesterase (AChE). An electrochemical biosensor which use small amount of enzyme have been developed for detecting AChE inhibitory activity. The biosensor used acetylcholine chloride (AChCl) as substrate for the enzyme-modified electrode, on which AChE is co-immobilized with inhibitor. AChE is sensitive for the inhibitor (including herbals). As a result, the current generated is inversely proportional to the amount of inhibitor present in the solution.