PENGEMBANGAN BIOSENSOR POLIFENOL BERBASIS POLIFENOL OKSIDASE (PPO) DAN 3-M3TIL-2-BENZOTHIOZOLINON HIDRAZAON (MBTH) UNTUK DETEKSI POLIFENOL PAFA PRODUK MINUMAN TEH DALAM KEMASAN

Abstract

The purpose of biosensor development is for polyphenols detection in beverage tea products especially green tea product easily and efficiently. The polyphenol biosensor has been made by immobilizing 5.0 µ l reagent (500 unit/ml PPO enzyme and 12.0 mg/ml MBTH (1:1 v/v) is mixture) on whatman filter paper. Polyphenol biosensor has been characterized and the results are polyphenol biosensor has response time about 13-18 minutes, has linearity at concentration range of 25-300 ppm, with correlation coefficient (r) =0.997. This polyphenol biosensor has sensitivity about 0.1078/ppm CE, while LOD = 20.601 ppm CE and LOQ = 68.671 ppm CE. Interference or selectivity of the polyphenol biosensor by catechin and vitamin C concentration ratio of 1:3,33 is 6.117% . Biosensor precision parameter comply with RSD 3.111% and the accuracy parameter with recovery about 103.068 %. Biosensor self-live is 8 days when strored on freezer [(-20)-(0) ºC]. This polyphenol biosensor has been applied to some beverage green tea product and this method is as good as Folin-Ciocalteu.