Uji Ketahanan Variasi pH dan Konsentrasi NaCl Bakteri Asam Laktat Asal Usus Halus Ayam Pedaging
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Fakultas Matematika dan Ilmu Pengetahuan Universitas Jember
Abstract
Lactic Acid Bacteria (LAB) are bacteria that produce lactic acid as the
main metabolite product through the fermentation process. LAB can be found in
the digestive tract of livestock, including the small intestine of broilers. Most LAB
species have been characterized as probiotic agents to improve the health and
productivity of broilers. One of the important requirements for LAB as a probiotic
is that it must be able to survive in extreme conditions in the digestive tract such
as acidic pH and high NaCl concentration. This study aims to obtain data on the
resistance of LAB isolates from the small intestine of broilers to acidic pH and
NaCl concentration.. This study was carried out through several stages, namely
rejuvenation of LAB isolates, pH resistance tests, NaCl resistance tests, and
calculation of LAB survival rates. The result of the study showed that of the 7
isolates, namely U10, U11, U13, U16, U31, U33, and U37, all isolates could
survive at pH 2,3,4 and NaCl concentrations of 2%, 4%, 6%, and 8%. Based on
the results, isolate U11 had the best resistance to acidic pH and high salt
concentrations compared to other isolates. Therefore, isolate U11 is the best
probiotic candidate that can be applied to broilers to improve their health and
productivity.
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