Uji Aktivitas Antidepresan Ekstrak Etanol Rimpang Temu Giring (Curcuma heyneana Val.) pada Mencit

Abstract

This study aimed to evaluate the antidepressant-like potential of an ethanol extract of temu giring rhizome (Curcuma heyneana Val.) in mice using an acute stress model. Mice were allocated into a control group receiving 1% CMC Na, a positive control group treated with fluoxetine, and treatment groups administered the ethanol extract at three dose levels (100, 200, and 400 mg/kg). All treatments were given orally prior to behavioral assessment. Antidepressant-like activity was evaluated by measuring immobility time in the Forced Swim Test (FST) and Tail Suspension Test (TST), where reduced immobility reflects a shift from passive to active coping. Serum cortisol levels were measured as a supporting indicator of physiological stress response. Data were analyzed using between-group comparative statistics followed by post hoc testing at a 95% confidence level. In the FST, the 200 mg/kg dose produced a significant reduction in immobility compared with the control and appeared to be the most consistent in promoting active coping. In the TST, treatment groups showed a downward trend in immobility; however, between-group differences were not statistically significant. Serum cortisol levels were significantly lower in all extract-treated groups compared with the control, with no significant differences observed among extract doses. Overall, the ethanol extract of temu giring rhizome demonstrates antidepressant-like potential in an acute stress model, with 200 mg/kg identified as an efficient dose based on behavioral outcomes, while further studies are required to clarify the underlying mechanisms.