Karakteristik Translokasi Bakteriofag Rekombinan ΦSen-NF-rec Penginfeksi Salmonella enterica dari Saluran Pencernaan ke Sistem Sirkulasi pada Tikus Putih

Abstract

Bacterial resistance to antibiotics (multidrug resistant, MDR) was a global health problem, including in Salmonella enterica serovar Salmonella typhimurium. Bacteriophages were considered a potential alternative therapy because they were specific to target bacteria. This study aimed to determine the presence of Sen-NF-rec Recombinant Bacteriophage in the digestive system and blood circulation system of white mice and to determine its concentration. The study was conducted using white mice that were orally infected with Salmonella typhimurium and treated with Sen-NF-rec Recombinant Bacteriophage via intraperitoneal injection, with eight treatment groups. Blood samples were collected at 5 and 27 hours after the first treatment, while intestinal samples were collected after three treatments. The presence of bacteriophages was analyzed using spot assay and plaque assay methods, and the results were calculated in PFU/mL units. The results showed that the Sen-NF-rec Recombinant Bacteriophage was not detected in blood samples, but was detected in the intestinal samples of white mice, particularly in the Prevention II (P5) and Treatment II (P7) groups, with average titers of 1.41 ± 0.48 × 10⁶ PFU/mL and 0.89 ± 0.18 × 10⁶ PFU/mL, respectively. This study showed that bacteriophages survived in the digestive tract after repeated exposure but were not detected in the blood circulation system under the conditions of this study.