Elevated Blood Serum Neutrophil Collagenase and NADPH Oxidase –1 (NOX–1) in Acute Coronary Syndrome

Abstract

Context: High–level neutrophil activity and oxidative stress were reported in Acute Coronary Syndrome (ACS). Aims: This study aimed to analyze serum level neutrophil collagenase (Matrix Metalloproteinase 8, MMP–8) and oxidative enzyme NADPH Oxidase–1 (NOX–1) in ACS. Settings and Design: This study an observational cross–sectional study. Methods and Material: A total of 84 subjects were studied, consisted of 52 ACS patients (including 21 unstable angina and 15 Non–ST–Segment Elevation Myocardial Infarction [NSTEMI] and 16 ST–Segment Elevation Myocardial Infarction [STEMI]), 16 subjects with stable angina pectoris and 16 healthy control. The level of serum MMP–8 and NOX–1 were analyzed by enzyme–linked immunosorbent assay (Elisa). Statistical analysis used: Data were analyzed using Anova and LSD. Results: Level serum MMP–8 and NOX–1 were significantly (p < 0.05) higher in ACS, the highest level of both markers were found in STEMI patients. Neutrophil leukocytosis was frequently found in STEMI patients as well. Conclusions: ACS patients demonstrated sign of inflammation and oxidative stress indicated by elevated serum neutrophil collagenase and NOX–1. These evidences might provide as markers for predicting as well as a guide for preventing and developing therapy for ACS.