A Novel Colorimetric Food Package Label for Fish Spoilage Based on Polyaniline Film
HENG, Lee Yook
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A novel colorimetric method based on polyaniline (PANI) ﬁlm for the development of smart packaging, as a chemical sensor for real-time monitoring of the microbial breakdown products in the headspace of packaged ﬁsh is described. This on-package indicator contains PANI ﬁlm, that responds through visible color change to a variety of basic volatile amines (speciﬁcally known as total volatile basic nitrogen (TVBN)) released during ﬁsh spoilage period. The PANI ﬁlm characteristics and its response to TVBN were studied. A kinetic approach was used to correlate the ammonia response of the PANI ﬁlm to that of the ﬁsh spoilage. Color changes, in terms of total color difference of PANI, correlated well with TVBN levels of ﬁsh. Apart from TVBN, trials on milkﬁsh sample (Chanos chanos) have veriﬁed that the PANI ﬁlm response also correlates well with microbial growth patterns in ﬁsh samples, especially the changing microbial populations (total viable count (TVC) and Pseudomonas spp.). These responses enabled the real-time monitoring of ﬁsh spoilage either at various constant temperatures or with temperature ﬂuctuations. The PANI ﬁlm can be recycled several times using an acid solution to regenerate the PANI surface. Thus, PANI ﬁlm can be considered as a low-cost sensor suitable for smart packaging applications.
- LSP-Jurnal Ilmiah Dosen