| dc.description.abstract | Bacterial blight disease caused by Pseudomonas syringae pv. glycinea becomes one among several important factors that affects
soybean production. To control this pathogen, most farmers apply synthetic pesticides that have negative effect for human,
animal, and environment. Therefore, an alternative control must be developed including the use of bacteriophages. Moreover,
identification and detection of this pathogen should be accurate for optimal diseases management. On the other hand,
bacteriophage, a virus that infects specific host-bacterium, is largely known to have the ability to kill their specific hostbacterium.
In
addition,
bacteriophage
is
also
used
as
diagnostic
tool
for
bacterial
detection
and
identification
of
purpose,
known
as
phatovar
classification.
This
research
was
aimed
to
isolate
the
bacterial
blight
pathogen
and
their
bacteriophage
from
soybean
field
in
Jember,
determine
the
pathogen
pathogenicity
and
virulence,
and
determine
the
host-range
of
bacteriophage
that
infects
several
isolates of bacterial blight pathogen on soybean. The results has shown that among 12 isolates of bacterial blight
pathogens that had similar characteristic to P. syringae pv. glycinea, only 10 isolates showed positive hypersensitive response
(HR) on tobacco leaf. However, all isolates were virulent when inoculated to soybean leaves by showing particular bacterial
blight symptom. In addition, one bacteriophage was found to infect isolate H3, called PSGH3, and was to have 6 hosts among
12 bacterial isolates including isolates H3, SK4-2, BT4-1, KR1-1, KR4-2 dan MG4-1. The results from plaque assay PSGH3
with ϕH3-1 found out three only particle bacteriophage which are ϕGH1, ϕGH2 and ϕGH3. | en_US |
