| dc.description.abstract | Background: Damage to bone tissue resulting from tooth extraction will cause alveolar bone resorption. Therefore, a material for
preserving alveolar sockets capable of maintaining bone is required. Hydroxyapatite Gypsum Puger (HAGP) is a bio-ceramic material
that can be used as an alternative material for alveolar socket preservation. The porous and rough surface of HAGP renders it a good
medium for osteoblast cells to penetrate and attach themselves to. In general, bone mass is regulated through a remodeling process
consisting of two phases, namely; bone formation by osteoblasts and bone resorption by osteoclasts. Purpose: This research aims to
identify the effects of HAGP scaffold application on the number of osteoblasts and osteoclasts, as well as on the width of trabecular
bone area in the alveolar sockets of rats. Methods: This research used Posttest Only Control Group Design. There were three research
groups, namely: a group with 2.5% HAGP scaffold, a group with 5% HAGP scaffold and a group with 10% HAGP scaffold. The number
of samples in each group was six. HAGP scaffold at concentrations of 2.5%, 5% and 10% was then mixed with PEG (Polyethylene
Glycol). The Wistar rats were anesthetized intra-muscularly with 100 mg/ml of ketamine and 20 mg/ml of xylazine base at a ratio of
1:1 with a dose of 0.08-0.2 ml/kgBB. Extraction of the left mandibular incisor was performed before 0.1 ml preservation of HAGP
scaffold + PEG material was introduced into the extraction sockets and suturing was performed. 7 days after preparation of the rat
bone tissue, an Hematoxilin Eosin staining process was conducted in order that observation under a microscope could be performed.
Results: There were significant differences in both the number of osteoclasts and osteoblasts between the 2.5% HAGP group, the
5% HAGP group and the 10% HAGP group (p = 0.000). Similarly, significant differences in the width of the trabecular bone area
existed between the 5% HAGP group and the 10% HAGP group, as well as between the 2.5% HAGP group and the 10% HAGP group
(p=0.000). In contrast, there was no significant difference in the width of the trabecular bone area between the 2.5% HAGP group
and the 5% HAGP group. Conclusion: The application of HAGP scaffold can reduce osteoclasts, increase osteoblasts and extend the
trabecular area in the alveolar bone sockets of rats. | en_US |
