| dc.description.abstract | Sucrose phosphate synthase (SPS; EC 2.3.1.14) plays an important role in partition of assimilated carbon in most plants. SPS
catalyses the penultimate reaction in the pathway of sucrose synthesis, in which sucrose-6-phosphate (Suc6P) is synthesized from UDPglucose
(UDPG)
and
fructose-6-P
(Fru6P).
To
increase
the
capacity
of
sugarcane
in
sucrose
synthesis,
spindle
leaves
of
sugarcane
cv
R579
were
transformed
with
cDNA
SoSPS1
from
sugarcane
under
the
control
of
constitutive
promoter
(35S
CaMV)
that
constructed
in pBI 121 (pKYS) using Agrobacterium tumefaciens. Based on PCR analysis, we have detected the existence of SPS transgene in
some lines of transformed sugarcane, called line 4, 5, 6, and 7. The SPS transgene in transformed sugarcane could be expressed into
translation level and increased the amount of leaves SPS protein, so the activity of leaves SPS was higher than wild type sugarcane
as control. The transformed sugarcane line 4, 5, 6, and 7 showed 1.4–2.9 fold increases in SPS activity and 1,76–2,2 fold increases in
leaves sucrose content. Increasing in SPS activity in transgenic sugarcane was coupled by the increase in invertase activity and ratio
between sucrose and starch content. | en_US |
