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dc.contributor.authorMiswar, Miswar
dc.contributor.authorSugiharto, Bambang
dc.contributor.authorSoedarsono, Joedoro
dc.contributor.authorMoeljapawiro, Sukarti
dc.date.accessioned2020-01-21T02:25:38Z
dc.date.available2020-01-21T02:25:38Z
dc.date.issued2007-02-24
dc.identifier.urihttp://repository.unej.ac.id/handle/123456789/97060
dc.description.abstractSucrose phosphate synthase (SPS; EC 2.3.1.14) plays an important role in partition of assimilated carbon in most plants. SPS catalyses the penultimate reaction in the pathway of sucrose synthesis, in which sucrose-6-phosphate (Suc6P) is synthesized from UDPglucose (UDPG) and fructose-6-P (Fru6P). To increase the capacity of sugarcane in sucrose synthesis, spindle leaves of sugarcane cv R579 were transformed with cDNA SoSPS1 from sugarcane under the control of constitutive promoter (35S CaMV) that constructed in pBI 121 (pKYS) using Agrobacterium tumefaciens. Based on PCR analysis, we have detected the existence of SPS transgene in some lines of transformed sugarcane, called line 4, 5, 6, and 7. The SPS transgene in transformed sugarcane could be expressed into translation level and increased the amount of leaves SPS protein, so the activity of leaves SPS was higher than wild type sugarcane as control. The transformed sugarcane line 4, 5, 6, and 7 showed 1.4–2.9 fold increases in SPS activity and 1,76–2,2 fold increases in leaves sucrose content. Increasing in SPS activity in transgenic sugarcane was coupled by the increase in invertase activity and ratio between sucrose and starch content.en_US
dc.language.isoInden_US
dc.publisherBerkala Penelitian Hayati, Vol. 12, No. 2, February 2007en_US
dc.subjectSoSPS1en_US
dc.subjectsugarcaneen_US
dc.subjectCaMVen_US
dc.subjectAgrobacterium tumefaciensen_US
dc.subjectpBI121en_US
dc.titleTransformasi Gen Sucrose Phosphate Synthase (SoSPS1) Menggunakan Agrobacterium tumefaciens untuk Meningkatkan Sintesis Sukrosa pada Tanaman Tebu (Saccharum officinarum L.)en_US
dc.typeArticleen_US
dc.identifier.kodeprodiKODEPRODI2520101#Magister Bioteknologi
dc.identifier.nidnNIDN0022105504


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