Development of Allergenicity and Toxicity Assessment Methods for Evaluating Transgenic Sugarcane Overexpressing Sucrose–Phosphate Synthase
Date
2019-01-08Author
Neliana, Intan Ria
Sawitri, Widhi Dyah
Ermawati, Netty
Handoyo, Tri
Sugiharto, Bambang
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Show full item recordAbstract
Sugarcane is considered as an industrial crop that produces sugar. The number of transgenic
sugarcane on the market is currently increasing. Therefore, investigation of the potential allergens
and toxics in transgenic sugarcane is necessary, since there is less information regarding food safety
for human consumption. Bioinformatics and experimental analysis were used for the validation of
the allergenic potential of transgenic sugarcane overexpressing sucrose–phosphate synthase (SPS).
Bioinformatics analysis showed that SPS has no homology with any known allergenic proteins.
However, eight-residues identical contiguous sequence was detected, and further specific assessment
is required to confirm the potential of allergenicity. The results of protein stability evaluation showed
that SPS gradually decreased at 28
C by heat treatment.
In addition, total protein was degraded by simulated gastric fluids (SGF), and simulated intestine
fluid (SIF) assays for one-minute incubation. The level of specific IgE in the transgenic sugarcane
and controls also showed no potential risk of allergy. An acute oral toxicity assay was performed
by oral gavage of transgenic sugarcane juice in mice. The LD50 for transgenic sugarcane juice was
>25 gr/kg body weight. We propose a development method for allergenicity and toxicity assessment
in transgenic sugarcane.
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- LSP-Jurnal Ilmiah Dosen [7292]