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dc.contributor.authorDewanti, Parawita
dc.contributor.authorSaputra, Bernet Agung
dc.contributor.authorWardiyati, Tatik
dc.contributor.authorSugiarto, Arifin Nur
dc.contributor.authorSugiharto, Bambang
dc.date.accessioned2019-12-12T03:12:50Z
dc.date.available2019-12-12T03:12:50Z
dc.date.issued2011-02-17
dc.identifier.urihttp://repository.unej.ac.id//handle/123456789/96753
dc.descriptionProceeding The International Conference on Basic Science 2011en_US
dc.description.abstractThe development of transgenic tomato to increase of sucrose translocation can be done through transformation by genetic engineering techniques. The process of sucrose transport from leaves to the stem/fruit facilitated by transport proteins (sucrose transporter/SUT). In the year 2008 has been isolated SoSUT1 gene to increase of the rate of sucrose transport, so the accumulation of sucrose in storage organs (stems/fruit/sinks) increases. Cotyledon and axillary bud from L. esculentum cv. Zamrud were co-cultivated with Agrobacterium strain GV3010 that harbored a pAct binary vectors carrying genes for both Hygromycin phosphotransferase (HPTII) and S. officinarum SPS (SoSUT1). This study aims to determine transformation and obtain transgenic tomato plants transformed with the SoSUT1 gene using Agrobacterium tumefaciens. Transformation efficiency was obtained at 10 mgl-1 hygromisin antibiotic as an selection agent with the percentage of explants forming shoots 6.2%. PCR results showed that the plasmid pKYS: SoSUT1 already integrated in the explant and has obtained five clones of transgenic tomato plants.en_US
dc.language.isoenen_US
dc.publisherUniversitas Brawijaya, Malang-Indonesiaen_US
dc.subjectTomatoen_US
dc.subjectSoSUT1 geneen_US
dc.subjectTransformationen_US
dc.subjectAgrobacterium tumefaciensen_US
dc.titleAgrobacterium Mediated Transformation of Tomato and Production of Transgenic Plants Containing Gene Increased Sucrose Transport SoSUT1en_US
dc.typeArticleen_US
dc.identifier.kodeprodiKODEPRODI1510501#Agroteknologi/Agroekoteknologi
dc.identifier.nidnNIDN0025046502


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