dc.description.abstract | The study aimed to identify variations in morphological and molecular
character of sugarcane mutants from the mutation of Ethyl Methane
Sulphonate (EMS). It used 21 sugarcane mutants and two non-mutant
PS865 plants as controls. The treatments with inundation were carried
out on 1-17 mutants and non-mutants, while the treatments without
inundation were carried out on 18-21 mutant plants and non-mutants.
The tolerance characteristics base on the agronomic characters.
The RAPD molecular character was observed to detect changes in
genotypes and kinship relationships of the plants tested. The results
showed that the characteristics of tolerance to the best inundation were
found in mutants 1, mutants 3 and mutants 6 which were characterized
by the root volume (cm3), fresh root weight (g), sucrose content and brix
value (%). Mutant plants treated with inundation showed higher levels of
sucrose and brix values. Whereas the control plants in fats, showed lower
levels of sucrose and brix. Changes in sugarcane mutant genotypes
from non-mutants based on RAPD markers ranged from 14.7 - 56.7 %
which resulted in an average polymorphic band of 35.1 % from 37 DNA
bands and produced four main groups based on dendrogram analysis. | en_US |