| dc.description.abstract | Introduction: Garlic is a natural herb which can be used to be a good alternative treatment
because cheap and safe. Garlic contains allicin which may has act antibacterial and antiinflammatory
effect. Moreover, garlic extract has a good biocompatibility and can stimulate
cell growth. Does garlic extract biocompatible and can stimulate cell growth that is seen from
the proliferation of human gingival fibroblasts and how its work will be studied. Objective: The
aim of this study was to analyze the biocompatibility of garlic extract by observing the viability
and proliferation of human gingival fibroblasts in vitro. Methods: Biocompatibility test was
conducted using serial concentration of garlic extract. Human gingival fibroblasts was seeded
into 96 microwell plate with density of 2x10
cells, added with the fourteen serial concentration
of garlic extract, and incubated in 37
o
3
C and 5% CO
for 24, 48 and 72 hours. MTT assay was
used to analyze the viability and proliferation of human gingival fibroblasts. Data were analyzed
by the Kruskal Wallis and U Mann-Whitney test. Results: The result showed that in each time
of observation, there is no significant difference in viability fibroblast (p>0,05), but there are
significant difference between time of observation at 24, 48, and 72 hours (p <0.05).Data
showed that all concentration of garlic extract increased the viability and proliferation of human
gingival fibroblasts. Conclusions: The ethanolic garlic extract has a good biocompatibility to
human gingival fibroblasts culture cell and can stimulate the proliferation of human gingival
fibroblast. | en_US |
