Potensi Proteolitik Isolat Bakteri SaPS 1 Asal Pantai Papuma, Jember

Abstract

Marine bacteria are a potential source of protease enzymes, a class of therapeutic protein used to treat various diseases, including cardiovascular disorders, cancer, and sepsis. This study was conducted to determine the proteolytic potential of the SaPS 1 bacterial isolate from Papuma Beach, Jember. The bacterial isolate was identified based on morphology and biochemical characteristics. Proteolytic activity was tested on bacterial colonies and crude protein extract using the casein plate assay on skim milk agar medium. The activity was evaluated based on the proteolytic index. Morphological characterization showed that the colonies were circular, dull white in color, undulate edges, and flat elevation. The bacterial isolate was Gram-positive and rod-shaped. Biochemical test showed positive results for the catalase test, starch and gelatin hydrolysis, while carbohydrate fermentation results cannot be interpreted. The proteolytic activity of SaPS 1 colony was low, indicated by the clear zone and proteolytic index of 0.60±0.15. This proteolytic activity was higher than that of Bacillus subtilis used as a positive control. The proteolytic activity of SaPS 1 crude protein extract, with a protein concentration of 1.933 mg/mL, showed a moderate level of activity, with a proteolytic index of 3.25±0.28. This proteolytic activity showed comparable results with Bacillus subtilis as a positive control. Based on these results, the crude protein extract isolated from the SaPS 1 bacterial isolate exhibits promising proteolytic activity and has the potential to be developed as a therapeutic protein.