| dc.description.abstract | Objective: This research aimed to evaluate the effect of fractionation of Merremia mammosa Lour. (Mm (Lour.)) extract on diabetic wound healing
by observing the collagen synthesis process and to search the most potent fraction.
Methods: Wistar rats were divided into five groups (n=5), i.e., K-(negative control), K+(positive control), K1 (ethyl acetate fraction), K2 (water
fraction), and K3 (n-hexane fraction). The Mm (Lour.) was extracted with ethanol 70%, then fractionated by using three solvents which have different
polarity. The rats were adapted in 7 d, then induced into diabetic by streptozotocin dose 40 mg/kg body weight. The wound was made by Morton
excision method. Treatment was given every two days and a skin biopsy was done on day 11. Analysis of collagen density was done by
photomicrograph of histopathology preparations in Masson’s trichome stained by using trinocular microscope with 400x magnification in 6 fields of
view, then processed by imageJ software and analyzed by appropriate statistic tool.
Results: The results of this research showed that fractionation of Mm (Lour.) extract significantly enhanced diabetic wound healing based on
macroscopic (percentage of wound healing) and collagen density with p-value<0.05 when compared with negative control, especially the water
fraction (p=0.000). The follow-up post hoc analysis showed that there was no significant (p=0.989) or there was no meaningful difference in the group
of water fraction when compared to positive control.
Conclusion: Water fraction is the extract fraction of Mm (Lour.) which has the most significant influence on diabetic wound healing showed by
enhancement of collagen synthesis. | en_US |
