Detection of Bacterial Wilt Pathogen and Isolation of Its Bacteriophage from Banana in Lumajang Area, Indonesia
Date
2019-02-22Author
Addy, Hardian Susilo
Azizi, Norita Fatatik
Mihardjo, Paniman Ashna
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Bacterial wilt disease on banana is an important disease in Lumajang District and causes severe yield loss. Utilizing bacteriophage
as natural enemy of pathogenic bacteria has been widely known as one of the control strategies. This research was aimed at
determining the causing agent of bacterial wilt on banana isolated from Lumajang area, to obtain wide-host range bacteriophages
against bacterial wilt pathogen and to know the basic characteristic of bacteriophages, particularly its nucleic acid type. Causative
agent of bacterial wilt was isolated from symptomatic banana trees from seven districts in Lumajang area on determinative CPG
plates followed by rapid detection by PCR technique using specific pair-primer. Bacteriophages were also isolated from soil of
infected banana crop in Sukodono District. Morphological observation showed that all bacterial isolates have similar characteristic
as common bacterial wilt pathogen, Ralstonia solanacearum. In addition, detection of FliC region in all isolates confirmed that
all isolates were R. solanacearum according to the presence of 400 bp of FliC DNA fragment. Moreover, two bacteriophages were
obtained from this experiment (𝜙RSSKD1 and 𝜙RSSKD2), which were able to infect all nine R. solanacearum isolates. Nucleic acid
analysis showed that the nucleic acid of bacteriophages was DNA (deoxyribonucleic acid).
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- LSP-Jurnal Ilmiah Dosen [7292]