| dc.description.abstract | Contaminating food-borne pathogen such as
Escherichia coli become one problem in food safety
especially for agricultural product. Detection of the
presence of pathogen may help customers to protect
themselves against food-borne disease. Several simple
techniques have been developing including the use of Total
Plate Count (TPC). However, this method is time consumed
resulting developing new approach in detecting E. coli
including the use of specific bacteriophage. This research
was aimed to isolate, formulate, and test the potency of
bacteriophage in detecting E. coli which is isolated from
post-harvest horticultural crops. About ten isolates were
isolated from 5 traditional vegetable markets in Jember
area. In addition, 2 kinds of bacteriophages were also
isolated from vegetables and sewage for ϕPst-1 and ϕKR-1,
respectively. Formulation of detection solution were based
on Luria Bertani Modified Medium (LBMM) composted by
LB + 1% Glucose (Glu) and 0.1% of Bromothymol Blue
(BTB) which was selected based on stability of green-color
at 570nm. Detection assay was also done using mixture of
detection solution, sample, and bacteriophage ϕPsT-1 which
was incubated at both room temperature and 37°C. The
result showed that, the kit was able to determine the
presence of E. coli about 4-5 hours depend on the
incubation temperature. Negative result was shown by no
color change while positive (contaminated by E. coli) result
shown by the changing of color from green to yellow.
Through this result we claimed that the technique is more
efficient than TPC methods. | en_US |
