| dc.description.abstract | Induction of somatic embryogenesis in sugarcane requires the composition Plant Growth Hormone
(PGH) appropriate. Utilizing of PGH (2,4-D and kinetin) is expected to induce sugarcane somatic
embryogenesis. The purpose of this study was to obtain the concentration of 2,4-D and kinetin that
effective for the multiplication of sugarcane var. NXI 1-3 through somatic embryogenesis. This study
consists of four stages: callus induction, callus proliferation, regeneration of shoots and encapsulation.
The plant material used is a spindle leaf sugarcane var. NXI 1-3. Callus induction used 2,4-D with
different concentration (2 ppm, 3 ppm and 4 ppm). Callus proliferation used 2,4-D with concentration 1
ppm and 2 ppm. Regeneration of shoots used kinetin 0,5 ppm. The results are showed that the optimal
induction of embryogenic callus on medium MS + sucrose 30 g / L + CH 300 ppm + 300 ppm PVP +
2,4-D 4 ppm as indicated by the high percentage of explants forming embryogenic callus that is equal to
40% and the respective concentration 2 ppm and 3 ppm is 33,3% and 37,5%. In proliferation stage, the
development callus optimal on medium MS + sucrose 30 g / L + CH 300pm + PVP 300 ppm + 2,4-D 2
ppm and formulations for regeneration shoot on medium MS + sucrose 30 g / L + kinetin 0.5 ppm. The
result of encapsulation can be shaped 100 sythetic seed. | en_US |
