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    RNA-Dependent RNA Polymerase 6 Is Required for Efficient hpRNA-Induced Gene Silencing in Plants

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    F. P_Jurnal_Wahyu Indra_RNA-Dependent.pdf (9.843Mb)
    Date
    2017-11-27
    Author
    Harmoko, Rikno
    Fanata, Wahyu Indra Duwi
    Jae Yong Yoo
    Ki Seong Ko
    Yeong Gil Rim
    Uddin, Mohammad Nazim
    Siswoyo, Tri Agus
    Seung Sik Lee
    Dool Yi Kim
    Sang Yeol Lee
    Kyun Oh Lee
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    Abstract
    In plants, transgenes with inverted repeats are used to in- duce efficient RNA silencing, which is also frequently induced by highly transcribed sense transgenes. RNA silencing induced by sense transgenes is dependent on RNA-dependent RNA polymerase 6 (RDR6), which converts single-stranded (ss) RNA into double-stranded (ds) RNA. By contrast, it has been proposed that RNA silencing induced by self-complementary hairpin RNA (hpRNA) does not require RDR6, because the hpRNA can directly fold back on itself to form dsRNA. However, it is unclear whether RDR6 plays a role in hpRNA-induced RNA silencing by amplifying dsRNA to spread RNA silencing within the plant. To address the efficiency of hpRNA-induced RNA silencing in the presence or absence of RDR6, Wild type (WT, Col-0) and rdr6-11 Arabidopsis thaliana lines expressing green fluorescent protein (GFP) were generated and transformed with a GFP-RNA interference (RNAi) construct. Whereas most GFP-RNAi-transformed WT lines exhibited almost complete silencing of GFP expression in the T1 generation, various levels of GFP expression remained among the GFP-RNAi-transformed rdr6-11 lines. Homozygous expression of GFP-RNAi in the T3 generation was not sufficient to induce complete GFP silencing in several rdr6-11 lines. Our results indicate that RDR6 is required for efficient hpRNA-induced RNA silencing in plants.
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    http://repository.unej.ac.id/handle/123456789/83360
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    UPT-Teknologi Informasi dan Komunikasi copyright © 2021  Perpustakaan Universitas Jember
    Contact Us | Send Feedback

    Indonesia DSpace Group :

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    Repository UIN Syarif Hidayatullah Jakarta