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    Abnormal Chloroplast Development and Growth Inhibition in Rice Thioredoxin m Knock-Down Plants

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    F. P_Jurnal_Wahyu Indra_Abnormal Chloroplast.pdf (1.726Mb)
    Date
    2017-11-27
    Author
    Yong Hun Chi
    Jeong Chan Moon
    Jin Ho Park
    Ho-Seung Kim
    Ismayil S. Zulfugarov
    Fanata, Wahyu Indra
    Ho Hee Jang
    Jung Ro Lee
    Young Mee Lee
    Sun Tae kim
    Yong-Yoon Chung
    Chae Oh Lim
    Jae-Yean Kim
    Dae-Jin Yun
    Choon-Hwan Lee
    Kyun Oh Lee
    Sang Yeol Lee
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    Abstract
    Plant cells contain several thioredoxin isoforms that are characterized by subcellular localization and substrate specificity. Here, we describe the functional characterization of a rice (Oryza sativa) thioredoxin m isoform (Ostrxm) using a reverse genetics technique. Ostrxm showed green tissue-specific and light-responsive mRNA expression. Ostrxm was localized in chloroplasts of rice mesophyll cells, and the recombinant protein showed dithiothreitol-dependent insulin b-chain reduction activity in vitro. RNA interference (RNAi) of Ostrxm resulted in rice plants with developmental defects, including semidwarfism, pale-green leaves, abnormal chloroplast structure, and reduced carotenoid and chlorophyll content. Ostrxm RNAi plants showed remarkably decreased F v /F m values under high irradiance conditions (1,000 mmol m ) with delayed recovery. Two-dimensional electrophoresis and matrix-assisted laser-desorption/ionization time-of-flight analysis showed that the levels of several chloroplast proteins critical for photosynthesis and biogenesis were significantly decreased in Ostrxm RNAi plants. Furthermore, 2-Cys peroxiredoxin, a known target of thioredoxin, was present in oxidized forms, and hydrogen peroxide levels were increased in Ostrxm RNAi plants. The pleiotropic effects of Ostrxm RNAi suggest that Ostrxm plays an important role in the redox regulation of chloroplast target proteins involved in diverse physiological functions.
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    http://repository.unej.ac.id/handle/123456789/83353
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    • LSP-Jurnal Ilmiah Dosen [7369]

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    Indonesia DSpace Group :

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