| dc.description.abstract | Previously we reported the identification of seven quantitative trait loci (QTLs) associated with
the rice yield measuring five parameters including panicles per plant (PPP), spikelets per panicle
(SPP), seed set percentage (SSP), 1000-grain weight (TGW) and yield in 2012. Here we report the
analysis of QTLs using the same trait parameters data of the mapping population in 2013 for detecting
highly
conserved
QTL
markers.
A
total
of
6
QTLs
were
identified
from
chromosomes
1,
7,
8,
10, 11, and 12, which were contrasted with our previous results (chromosomes 1, 2, 4, 5, 6, 8, and
11). In this comparison, three QTLs from chromosome 1, 8, and 11 were only found to be associated
with
the
components
of
yield
over
two
consecutive
years
indicating
high
sensitivity
of
QTL
markers to the environment. Of those three QTLs, SPP-associated marker RM12285 was found to
be dominantly expressed by real-time PCR (qPCR). In addition, compared to our previous report
the numbers of mapping population and markers were significantly increased for higher resolution
markers
from
70
to
120,
and
from
143
to
217,
respectively.
We
also
found
that
the
parameter
SPP
was
dominantly
correlated
with
the
rice
yield.
Furthermore,
the
double
haploid
(DH)
population
facilitated
to
analyze
the
epistatic
effects
for
yield
and
yield
components
in
rice.
Taken
together,
combining
multiple
mapping
population
data
over
years
possibly
enables
narrowing
down
to
the
highly
conserved
QTL
markers
against
diverse
environmental
fluctuation
caused
by such
as
drought
and
high
temperature.
Thus,
these
data
would
be
critically
exploited
to
improve
for
the
crop
breeding
strategy. | en_US |
