| dc.description.abstract | SCMV (Sugarcane Mosaik Virus) is sugarcane crop disease resulting in chlorosis in leaves with the formation of
the colors yellow and green intermittents. Based on survey information obtained that the air observation of all
varieties of sugarcane was already stricken with the virus SCMV. Even the most formidable attack is on PS881
varieties with the intensity of the attacks reached 80%, so that it is estimated will lose up to 40% of the harvest.
The sugarcane is virus free can be obtained via organogenesis in tissue culture method directly on the apical
meristem, somatic embryogenesis at, and also with the addition of khemoterapeutan (acyclovir).The workings of
the khemoterapi materials are the chemotherapi will interfere with replication and synthesis of genetic material
of the virus but also cause the same effect against the mechanism of synthesis of nuklet acid on plants hosts. This
research aims to find the best concentration and exposure time the most good in eliminating viruses SCMV in
the apical bud culture of sugarcane PS881, using the antiviral acyclovir in conditions of invitro, so that the
resulting plant will be virus free. The methods used to detect the presence of the virus by using the two ways, the
first is by serology test through the protein content of the virus / checking nucleic acid virus with ELISA and the
second is by RT-PCR. The results of the analysis showed that the interaction between the concentration of
khemoterapi and exposure time produces the best treatment in eliminating the virus was . treatment with
acyclovir concentration of 20 ppm and exposure time of 5 weeks. | en_US |
