Aktivitas Fagositosis Monosit yang Diinkubasi Ekstrak Daun Kopi Robusta (Coffea Robusta) dan Dipapar Candida albicans (Phagocytic Activity of Monocyte Incubated in Robusta Coffee Leaves Extract and Exposed by Candida Albicans)

Abstract

The role of C. albicans as pathogen opportunistic in oral infection can affected by immune system. Phagocytosis has contributed in immune system against C. albicans infection, which is the role of monocytes. Coffee has been proved to increase monocyte’s phagocytic activity, but the effect of robusta coffee leaves extract (EDKR) to increase monocyte’s phagocytic activity on C. albicans is unknown. Objectives:To determine the effect of coffee robusta leaves on monocyte’s phagocytic activity on C. albicans. Methods: This research was acted invitro on human monocyte cell culture. There was 5 groups: negative control group, positive control group (incubated in Isoprinosine) and treatment groups was incubated in EDKR 2,5%, 5%, 10%. The percentage of activated monocytes was counted. Result: The result showed that incubation of EDKR 2,5%, 5% and 10% could increase monocyte’s phagocytic activity significantly (p<0,05) compared to control groups. The number of monocyte’s phagocytosis on C. albicans incubated with EDKR 2,5%, 5% and 10% is 65%, 60,5%, and 58,75%. The optimal concentration of EDKR to increase monocyte’s phagocytic activity was 2,5%. Conclusion: The study showed that EDKR can increase monocyte’s phagocytic activity on C. albicans.