| dc.description.abstract | Elimination Sugarcane Mozaic Virus (SCMV) in sugarcane plantlets and propagation via somatic embryogenesis was assessed.
The aim of this study was to obtain a virus-free sugarcane and determine methods for propagation via somatic embryogenesis.
Explants derived from sugarcane SUT-02 plantlets. The explants were cultured on ribavirin and acyclovir medium at 0, 20 and 40
mgl
-1
2,3)
concentrations. Virus-free plantlets detected by DAS-ELISA. Rapid propagation virus-free plantlets through somatic
embryogenesis consists of 3 stages. Stage 1: callus induction on the media (a) MSo, (b) MSo + 3 mgl
-1
2,4-D, (c) MSo + 4 mgl
2,4-D, (d) MSo + 3 mgl
-1
2,4-D + 1.5 mgl
-1
BAP, (e) MSo + 4 mgl
-1
2,4-D + 1.5 mgl
-1
BAP, stage 2: proliferation on media MSo
+ 1.5 mgl
-1
2,4-D and stage 3: regeneration on MSo media. The results showed that the media ribavirin and acyclovir at 20 mgl
and 40 mgl
-1
concentration produces 100% virus-free sugarcane plantlets. Best embryogenic callus induction media is MSo + 3
mgl
-1
2,4-D and virus free plantlets have produced as much as ± 35 plantlets within 9 weeks. | en_US |
