Validation of Spectrophotometric Method For Estimation of Eperisone Hydrochloride in Tablet Dosage Form

Abstract

A simple and reproducible UV-spectrophotometric method was developed for the determination of Eperisone Hydrochloride in tablet. The absorbance were measured at 255.5 nm as the maximum absorbance of Eperisone Hydrochloride in methanol. Method was found linear over the concentration range of 1-18 µg/ml with the correlation coefficient of 0.999. The limit of detection (LOD) and the limit of quantification (LOQ) of the method were 0.458 µg/ml and 1.373 µg/ml. The precision of this method was less than 2% whereas the means of the recovery data were 100.94% ± 0.81. The proposed method has been applied to the determination of Eperisone Hydrocloride in commercial tablet formulations and the recovery of label claim were 98.84% ± 0.32 µg/ml for brand A and 99.74% ± 0.47 µg/ml for brand B.