GOAT KEFIR STIMULATE THE SHIFTING OF TH2 CELLS IN HEALTHY PERSON

Abstract

Kefir, one of fermented milk product consist of a mixture of yeast and bacteria has been known to promote health. Several studies on mice demonstrated that kefir may play a role as immunomodulator. However only few of them explore how kefir stimulate immune cells as well as cytokines that involve in innate immune system directly in human. This study aims to observe the immunomodulatory effect of kefir in vitro in healthy individuals. We measured the lymphocytes T CD4 + and CD8 + cells and secretion of Th1 and Th2 cells cytokine. We used kefir from goat milk with maximal age of 1 month after fermentation process. Eleven Volunteers were recruited after being informed about the study and signing informed consent. Peripheral Blood Mononuclear Cells (PBMCs) were isolated from 12 ml blood of normal healthy individuals. As many as 5.10 5 cells were cultured after subjected with addition different concentration of kefir: 5% (P1), 2% (P2), 1% (P3) and 0.5% (P4). PPD served as positi ve control (K+). Percentages of CD4 + and CD8 + T lymphocytes were measured by flowcytometer. Expression of IL2

and IL-10 levels were determined by ELISA method. The measurement of CD4 T cell demonstrated that there was no difference in both groups (p = 0.178 and p = 0.438) compared to K- as well as K+. In contrast the IL-2 levels showed significantly different between K- with K+ (p = 0.002), K+ with P2 (p = 0.002), P3 (p = 0.001) and P4 (p = 0.007). Finally the concentration of IL-10 were significantly different between K- with K+ (p = 0.008), K- with P1 (p = 0.004), P2 (p = 0.006), P3 (p = 0.004) and P4 (p = 0.017). Low concentration of kefir apparently modulate immune system of healthy person by shifting the immune response towards Th-2 cells through increasing level of IL-10.