| dc.description.abstract | Humans develop anti-salivary proteins after arthropod bites or exposure
to
insect salivary proteins. This reaction indicates that vector bites have a positive
effect
on the host immune response, which can be used as epidemiological markers
of
exposure to the vector. Our previous study identified two immunogenic proteins
with
molecular weights of 31 kDa and 56 kDa from salivary gland extract (SGE) of
Aedes
aegypti that cross-reacted with serum samples from Dengue Hemorrhagic
Fever
(DHF) patients and healthy people in an endemic area (Indonesia). Serum sam-
ples
from
individuals living in non-endemic area (sub-tropical country) and infants
did
not
tify
two
show
the immunogenic reactions. The objective of this research was to iden-
immunogenic
proteins, i.e., 31 and 56 kDa by using proteomic analysis. In
this
study, proteomic analysis resulted in identification of 13 proteins and 7 proteins
from
the 31 kDa- and 56 kDa-immunogenic protein bands, respectively. Among those
proteins,
the D7 protein (Arthropode Odorant-Binding Protein, AOBP) was the most
abundant
in 31-kDa band, and apyrase was the major protein of the 56-kDa band. | en_US |
