| dc.description.abstract | Naringenin (NAR), a natural flavonoid aglycone of naringin
has been extensively investigated for its pharmacological
activities, including anti-tumor effects. However, its poor
bioavailability has been identified as the single most important
challenge in oral drug delivery. Based in this condition, it is used
nanoencapsulation to increase the effectiveness of NAR as anticancer.
The objectives of this research were to develop the
formulation of NAR-loaded nanoparticles (NARNPs) as well as to
evaluate its potential as anti-cancer against T47D breast cancer
cells line. NARNPs is prepared through the method of ionic
gelation, meanwhile its characteristic is evaluated through photon
correlation spectroscopy (PCS), transmission electron microscopy
(TEM), fourier transform infra-red spectroscopy (FTIR), and
different scanning calorimeter (DSC). The result of MTT test and
cellular uptake indicate that NARNPs increase cytotoxicity and
internalization of NAR to the cells compared to that of free NAR.
The result of qualitative apoptosis study using fluorescence
microscope indicates that both free NAR and NARNPs were able to
induce apoptosis. It can be conclude that Chitosan nanoparticles–
TPP conjugates have the capability to encapsulate naringenin
hence increase the cellular uptake and cytotoxcicity of naringenin
against T47D cell line. NARNPs also could induce the apoptosis
effect. | en_US |
