Respon Inflamasi Pulpa Gigi Tikus Spraque dawley setelah Aplikasi bahan Etsa Ethylene Diamine Tetraacetic Acid 19% dan Asam Fosfat 37%
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Etching agents such as ethylene diamine tetraacetic acid (EDTA) and phosphoric acid which are widely used in adhesive restoration system are aimed to increase retention of restorative materials; however, these agents may induce inflammation of dental pulp. The major function of the inflammatory response is to clear invading pathogens or damaged tissue/cells and therefore, initiate repair. Neutrophils and macrophages are motile phagocytes that constitute the body's first line of defense. The purpose of the present research was to study the effect of 19% EDTA and 37% phosphoric acid application as an etching agents on the inflammatory response of the dental pulp. Forty-five male Sprague Dawley rats were divided into 3 groups. Cavity preparation was made on the occlusal surface of maxillary first molar using a round diamond bur. 19% EDTA, 37% phosphoric acid, and distilled water were applied on the surface of the cavity of the teeth in group I, II, and III subsequently. The rats were sacrified at 1, 3, 5, 7, and 14 days after the application (n=3 for each day). The specimens were then processed histologically and stained with hematoxylin eosin. Anova showed a significant difference (p<0.05) among treatment groups, indicating that etching agents application induced neutrophils, macrophages and lymphocytes infiltration in the dental pulp. Tuckey HSD test showed that application of 37% phosphoric acid increased higher number of neutrophils, macrophages and lymphocytes significantly than 19% EDTA (p<0.05). In conclusion, 37% phosphoric acid induced higher number of the inflammatory cells than 19% EDTA.