Level of Immunoglobulin G Saliva in Aggressive Periodontitis Rat Induces with Porphyromonas Gingivalis ATCC 33277

Abstract

Porphyromonas gingivalis, (P. gingivalis) is a gram-negative anaerobes that involved in the pathogenesis of aggresive periodontitis. This organism has a variety of virulence factors, such as lipopolysaccharides. The colonization of bacteria in gingival tissue is considered to be the first step in the phatogenic process of aggressive periodontitis resulting in tissue destruction.High levels of P. gingivalis-spesific immunoglobulin G (IgG) are commonly observed, but there have not a variable host response to this organism appears to exist yet. Immunoglobulin G responses in adult periodontitis patients play role in colonization inhibition or eliminatin of P.gingivalis.The purpose of the present study was detection of IgG levels in saliva of aggressive periodontitis rats model that induced by P. gingivalis ATCC 33277. Methods: This study used male wistar rat samples and is divided into two groups. Group I is control or without treatment; Group II is rats that induced by P.gingivalis ATCC 33277 3 times a week for 2 month. The samples were observed by ELISA. Furthermore, the data were analyzed by T- test Results: The result show that Ig G levels in saliva increased in both of the groups; statistically showed there was significant difference between group I and II (p<0,05). Conclusion: Levels of Immunoglobulin G antibodies to P. gingivalis increased in saliva of aggressive periodontitis rats models. It can be helpful to the diagnostic in periodontal diseases.