PENGARUH EKSTRAK TEMPE TERHADAP APOPTOSIS DAN PROLIFERASI SEL KANKER PAYUDARA PADA TIKUS PUTIH GALUR WISTAR YANG DIINDUKSI DMBA

Abstract

Cancer is the leading cause of death worldwide and breast cancer is second rank of largest cancer in the world. Breast cancer ranked first on the patients hospitalization throughout hospital in Indonesia and ranked second in East Java. Breast cancer require high costs, for both preventions and treatments.. Tempe contain isoflavones that suspected be able to induce apoptosis and inhibit proliferation of breast cancer cells. Therefore this research needs to be done to find out the effect of tempe as breast cancer prevention through increasing apoptosis and decreasing proliferation of breast cancer cells. The objective of this research is to examine and analyze the effect of tempe extract to increasing apoptosis and decreasing proliferation of breast cancer cells in DMBA-induced rats strain Wistar and to know the effective doses of tempe extract. This research is a true experimental design study using randomized posttest control group design with completely randomized design. Research done to Rattus norvegicus strain Wistar induced DMBA and given tempe extracts according to the oral dose per group. The number of apoptosis and proliferation of calculated and analyzed with MANOVA test (Multivariate Analysis of Variance) and Simple Linear Regression test. The results is there are significant differences effect of giving tempe extract against apoptosis and proliferation of breast cancer cells in all dose groups. Dose of 20 mg/kgBW has a significant differences with the other treatment group. The results showed the most effective dose is the dose of 20 mg/kgBW.