Diskriminasi Daun Gandarusa (Justicia gendarrusa Burm.f.) Asal Surabaya, Jember dan Mojokerto Menggunakan Metode Elektroforesis

Abstract

Gandarusa leaf (Justicia gendarcusa Burm.f.) has been used traditionally by the Papuan indigenous people for the male contraception remedy. This plant has been further explorated to determine the mechanism of its reported activity. It was known that the activity through its inhibition on sperm hyaluronidase enzyme which was competitive and reversible. This plant has been undergoing phase Il-clinical trial in Surabaya, Indonesia for the non hormonal male contraceptive remedy since 2009. The gandarusa samples was harvested from East Javaarea: Surabaya, Jember and Mojokerto. As a part of the standardization of raw material, the protein profiling has been done to seek any proteins which can be used as a marker for the gandarusa leaf. The proteomic study was performed by one and two dimentional SDS PAGE Q e, 2-D). The area of protein spots was measured and compared using ImageJ program. The results showed that all three samples of gandarusa had relatively similar 1-D profiles. In this regard, the 2-D profiles were made to see the difference of their proteomics. Discrimination of gandarusa samples were made based on the availability of individual protein spot, and the color intensity of the same protein spot in all samples. It can be suggested that2-DE profiles can be used to differentiate the gandarusa samples.