Pengaruh Metode Ekstraksi Refluks dan UAE terhadap Kadar Senyawa Tilirosida Daun Jati Belanda (Guazuma ulmifolia Lamk)

Abstract

Jati Belanda (Guazuma ulmifolia Lamk) is one of the medicinal plants that for a long time has been used in traditional medicine. This plant contains various bioactive compounds, including flavonoids, phenolic acids, and terpenoids. Tiliroside, which is a marker compound in Jati Belanda, has many biological activities such as antioxidant, anti-obesity, anti-diabetic and anti-hypertensive. This study investigates the yield of tiliroside, a flavonoid compound, from the leaves of Jati Belanda (Guazuma ulmifolia Lamk) using two extraction methods: Reflux and Ultrasound-Assisted Extraction (UAE). The effects of extraction time, ethanol concentration, and solvent-to-simplisia ratio on the yield of tiliroside were optimized. The results showed that the UAE method with an ethanol concentration of 70%, an extraction time of 90 minutes, and a solvent-to-simplisia ratio of 1:5 resulted in the highest yield of tiliroside (5.493 ± 0.248 mg/g). The reflux method also showed a significant yield of tilirosid, but it was lower than that of the UAE method. The study suggests that the UAE method is a more efficient and effective method for extracting tiliroside from Jati Belanda leaves. The findings of this study can be used to optimize the extraction process of tiliroside, the marker compounds from Jati Belanda which has potential applications in the pharmaceutical and food industries