Efektivitas Nanopartikel Perak sebagai Antikontaminan dalam Sterilisasi Eksplan dan Media pada Kultur in Vitro Tanaman Porang (Amorphophallus muelleri)

Abstract

Propagation of porang plants can be carried out without being constrained by the season using in vitro culture propagation techniques, but propagation using in vitro culture techniques is still constrained by the sterilization process. Sterilization of in vitro culture explants is currently still not optimal and uses a variety of materials which have relatively high toxicity. A solution is needed for the problem of sterilization in in vitro culture in the form of using effective and efficient sterilizing materials, and does not damage the tissue in the explants. Silver nanoparticles are known to have the ability as an anticontaminant by inhibiting bacterial replication and not damaging the tissue in the explants. This study aims to determine the effect of the combined use of AgNPs on the media, concentration, and soaking time of the explants in the sterilization of porang field leaves. The factors observed in this study were the combination of AgNPs in the media, concentration, and immersion time of the explants with 24 levels repeated 3 times, so there were 72 experimental units. The results showed that the combination of the use of AgNPs in the media, concentration, and immersion time of the explants in the sterilization of porang field leaves had an effect with different tendencies. The K6 treatment (5 minutes, 100 ppm + 2 ppm media) had the best trend in terms of percentage of sterile explants and survival. The treatment with the best trend was shown by the K18 treatment (10 minutes, 100 ppm + 2 ppm media) which did not cause contamination and produced callus with 100% percentage and 100% roots with an emergence time of 34 DAP for callus and 50 DAP for roots.