Browsing LSP-Jurnal Ilmiah Dosen by Author "Nakagawa, Atsushi"
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Identification of UDP-glucose Binding Site in Glycosyltransferase Domain of Sucrose Phosphate Synthase from Sugarcane (Saccharum officinarum) by Structure-based Site-directed Mutagenesis
Sawitri, Widhi Dyah; Afidah, Siti Nurul; Nakagawa, Atsushi; Hase, Toshiharu; Sugiharto, Bambang (Biophysical Reviews (2018) 10:293–298, 2017-12-08)Sucrose phosphate synthase (SPS) is believed to be the key enzyme for controlling the biosynthesis of sucrose. SPSs consist of a functional glycosyltransferase domain that shares conserved residues with the glycosyltransferase ... -
Purification and Characterization of Recombinant Sugarcane Sucrose Phosphate Synthase Expressed in E. coli and Insect Sf9 Cells: An Importance of the N-terminal Domain for an Allosteric Regulatory Property
Sawitri, Widhi Dyah; Narita, Hirotaka; Ishizaka-Ikeda, Etsuko; Sugiharto, Bambang; Hase, Toshiharu; Nakagawa, Atsushi (J. Biochem. 2016;159(6):599–607, 2016-01-30)Sucrose phosphate synthase (SPS) catalyses the transfer of glycosyl group of uridine diphosphate glucose to fructose-6-phosphate to form sucrose-6-phosphate. Plant SPS plays a key role in photosynthetic carbon ...