| dc.description.abstract | Klebsiella pneumoniae is a Gram‑negative bacterium that is mostly
found as a nosocomial disease in hospitals. This bacterium is the largest contributor
to the number of causes of pneumonia. Immune responses that play a role against
K. pneumoniae is interferon‑gamma (IFN‑γ) as cellular immune responses,
which function on the mucosal barrier in response to infection and contribute to
the initial defense against various pathogens. The purpose of this study was to
determine the effect of exposure to K. pneumoniae pili protein 65.5 kDa on IFN
levels in mice. Methods: Thirty BALB/C mice were divided into three groups: K1
given phosphate buffer saline, K2 given pili protein and Freund’s adjuvant, and
K3 given Freund’s adjuvant. The measurement of IFN‑γ levels was carried out by
enzyme‑linked immunosorbent assay, the results of which were analyzed using the
Kruskal–Wallis. Results: Pili protein 65.5 kDa K. pneumoniae showed a positive
hemagglutination test with the highest titer of 1/8, which was indicated by the
absence of red aggregate dots at the bottom of the well. Analysis test showed that
the highest concentration of IFN‑γ levels was in the control group and the lowest
IFN‑γ level was found in the adjuvant group. Conclusion: Pili protein 65.5 kDa
K. pneumoniae had a role as a hemagglutinin protein and there was no effect of
K. pneumoniae pili protein 65.5 kDa on IFN‑γ levels in mice. | en_US |
