Sibling Analysis Using Mitochondrial Dna Displacement Loop (Mtdna D-Loop) Region in the Identification of Madurese Population
Date
2022-04-05Author
YUDIANTO, Ahmad
SOSIAWAN, Agung
PALUPI, Retno
NOVITA, Masniari
Metadata
Show full item recordAbstract
Analyzing the variations of nucleotide sequences in D-loop regions mtDNA can
determine the identity of specific individuals or populations and maternal kinship. The
Madurese population is an ethnic group that maintains its traditional customs in harmony
with the religions they adhere to and endogamous marriage as their marriage pattern. This
study applied PCR amplification and sequencing strategies on HVS-1 143 bp (nt 16268-16410) and HVS 2 126 bp (nt 34-159) of mtDNA D-loop regions. This study used buccal
swab samples collected from 50 pure Madurese families consisting of a mother and two
children. The homology analyses of female-female, male-female, and male-male siblings
showed 11 variants or morphs in 126bp HVS-2 D-Loop mtDNA (nt 34-159). The highest
variant were: female-female siblings (129GC: 15%), male sibling (120C A: 11.5%) -female sibling (120C: A: 11.5%), and male-male siblings (131TC: 11.5). The homological
analysis of female-female and male-female siblings showed 11 variants on 143bp HVS-1
mtDNA D-Loop (nt 16259-16410). The highest variants in female-female siblings were:
16387AG, 16387AC: 15%; male sibling-female sibling: 16393CT, 16393CA:
11.5%; while the homological analysis of male-male siblings showed 13 variants with the
highest percentage: 16367AG, 16367AC: 11%.
Collections
- LSP-Jurnal Ilmiah Dosen [7292]