dc.description.abstract | The effect of botanical pesticides as an insect pest control agent has been proven in
several studies. Despite capable of controlling pests, botanical pesticides have not been
reported to promote plant growth. One technique for making botanical pesticides served in dual
function is to combine them with beneficial microbes. This study was aimed to determine the
compatibility of 3 isolates of Bacillus sp. and 3 isolates of Pseudomonas sp. against botanical
pesticides. The botanical pesticides were made from a mixture of Ageratum conyzoides, Aglaia
odorata, and Azadirachta indica leaves. The solvent used 96% ethanol, and the process of
making botanical pesticides was performed using a rotary evaporator at 45˚C. The
compatibility test was performed in vitro by dropping 10 µl of botanical pesticides on the
bacterial culture. Tests were carried out using various concentrations, namely 0%, 25%, 50%,
75%, and 100%. The test results showed that Bacillus sp. A and Pseudomonas sp. E strain
were compatible on all assay concentrations. Bacillus sp. A and Pseudomonas sp. D and
Pseudomonas sp. E were compatible with the concentrations of 0 to 75%. Furthermore,
Bacillus sp. B, Bacillus sp. C, and Pseudomonas sp. F were compatible with the assay
concentrations of 0 to 50%. | en_US |