Pengaruh Penambahan Tepung Daun Kelor [Moringa Oleifera] Terhadapkadarprotein, Kalsium, dan Daya Terima Nugget Ikan Lemuru [Sardinella Lemuru]
Date
2022-07-02Author
ALAMI, Eqiel Navadz Akthar
SULISTYANINGSIH, Erma
KUSUMA, Irawan Fajar
RACHMANIA, Sheilla
Metadata
Show full item recordAbstract
Interaction of Cysteine-rich Interdomain Region (CIDR)α-Plasmodium falciparum Erythrocyte
Membrane Protein (PfEMP1) and Endothelial Cell Receptors especially CD36 on host cells is main
malaria pathogenesis, makes this domain as a malaria vaccine candidate. Recently, the development
of the malaria vaccine is conducted by recombinant technology, and the purification of the CIDRαPfEMP1 recombinant protein is a pivotal step. This study aimed to determine an optimal condition
to purify the CIDRα-PfEMP1 recombinant protein by affinity chromatography through imidazole
and NaCl concentration. The purified recombinant protein was visualized using SDS-PAGE and its
concentration was measured using Image J software and Bradford Assay. The data were analyzed
using SPSS 26 software, and the Paired T-Test analysis was conducted to compare the concentration
of purified recombinant protein from two different methods. The result showed that thetarget band
of purified recombinant protein was 27 kDa. The thickest target protein band was observed in
purified recombinant protein using 140 mM imidazole and 300 mM NaCl. The recombinant protein
concentration using Image J software was 0.025 µg/µL, while the Bradford Assay was 0.56 µg/µL.
The Paired T-Test analysis has a significance value of 0.010 (p<0.05), meaning there was a
significant difference between the concentration measurement using Image J software and Bradford
Assay. In conclusion, the optimized condition to purify the CIDRα-PfEMP1 recombinant protein by
affinity chromatography was using 140 mM imidazole and 300 mM NaCl. It is suggested to measure
the purified CIDRα-PfEMP1 recombinant protein concentration using the Bradford Assay method
due to its convenience and sensitivity.
Collections
- LSP-Jurnal Ilmiah Dosen [7292]